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Our knowledge of the signalling cascades involved in the control of embryo development is incomplete and may be related to osmotic signalling. We have tested whether the endoplasmic reticulum calcium pump (SERCA), which transfers Ca2+ from the cytoplasm into the endoplasmic reticulum, is involved in the osmotic signalling of the early embryo. SERCA activity was measured using the thapsigargin-sensitive ATPase assay, and the mRNA levels for SERCA were measured by RT-PCR. Embryos were cultured under three different concentrations of thapsigargin (0, 1, 2.5 microM) for 48 h. A decrease in SERCA activity was recorded with thapsigargin at concentrations of 1 and 2.5 microM. Reduced ER Ca2+ levels are known to inhibit calmodulin-stimulated SERCA activity. Thus, thapsigargin could therefore inhibit osmo-stimulated Ca2+-dependent kinase activity in the early embryo. In addition, thapsigargin reduced the size of the blastocyst and altered the pattern of cell division in the trophoblasts. At 2.5 microM, thapsigargin also reduced total cell number (2-3 cells at the morula stage and 3-4 cells at the blastocyst stage). These results suggest that SERCA activity is involved in the osmotic signalling of the pre-implantation embryo.2.5 GHz bulk acoustic wave oscillator based on a SiO2/Si3N4/SiO2 film.
The effect of SiO2/Si3N4/SiO2 thicknesses on 2.5 GHz bulk acoustic wave oscillations in silicon based resonators was analyzed experimentally and numerically. The analysis showed that a thickness of 5-10 nm is enough to achieve low power consumption and a large dielectric quality factor Q. The experimental results also confirmed that the quality factor of SiO2/Si3N
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