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The experimental findings in metastatic cell lines are in sharp contrast to the *in situ* data in tumour samples, where a homogenous distribution of *de novo* CpG hypermethylation is associated with high-stage disease. Interestingly, profiling *in situ* of histological stages of breast cancer showed increased methylation in DNA derived from a more advanced stage, consistent with the proposed role of aberrant methylation in late tumour stages \[[@B42],[@B46]\]. In our study, we also investigated if methylation of *CDH13* had a tendency to increase in more advanced disease, as reported in the study of Cox *et al.*\[[@B41]\], but did not find such an association. In the light of the results we obtained for *CDH13*, a study focusing on the association between *CDH13* hypermethylation and the number of tumour-initiating cells is warranted in order to unravel the role of hypermethylation.
To conclude, we investigated a novel tumour suppressor gene (*CDH13*) and found that hypermethylation in primary breast tumours was associated with lower gene expression. This could be due to DNA methylation of the promoter of the *CDH13* gene, which leads to reduced gene expression. These results from the study of a comprehensive set of breast tumour samples can be considered valid for individual cases, but not for groups of patients.
Abbreviations
=============
(DMSO): Dimethyl sulfoxide; (DNMT): DNA methyltransferase; (IHC): Immunohistochemical analysis; (MSP): Methylation-specific polymerase chain reaction
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DESCRIPTION
Bedrooms
4
Bathrooms
3
People
5
Sleeps
8
Owner
Georgie
Member Since 2017
Speaks:
english, french, german, spanish
Calendar last updated:
Dec 20, 2018
Amenities
Swimming pool
TV
Satellite or Cable
Children Welcome
Internet
Heater
Parking
Bathrooms
3 Bathrooms
Bathroom 1
toilet, shower, tub
Bathroom 2
toilet, shower, tub
Bathroom 3
toilet, shower
Bathroom 4
toilet, shower, tub
Outside
Terrace
Suitability
Wheelchair Accessible
Location Type
Beach
Meals
Guests provide their own meals.
Notes
The apartment is on the 2nd floor of the building, the rent is 50 euros, the apartment has all the amenities you need. It is located right on the sea, just walk and you are there. Next to the apartment is a supermarket (IG), a small bakery with breakfast, but all the products you need for your stay. In the supermarket you will find the basics, the rent to pay (soup, grocery,…) is 50 euros per week
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