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The expression of the murine tumor antigen LM-7 was investigated in a series of chemically transformed and spontaneously transformed mouse and rat cells. LM-7 is the LM7 monoclonal antibody of Abou-Kassem et al. (Proc. Natl. Acad. Sci. U.S.A., 80, 3508-3512, 1983), which reacts with a cell membrane component expressed by different mouse and rat neoplastic cells, but not by normal cells. The expression of LM-7 antigen is lost on immortalization of normal cells. The regulation of LM-7 antigen expression was analyzed at the level of transcription and post-transcription. The following results were obtained: The LM7 gene was transcribed at a low level in normal cells, and its transcription rate was elevated in chemically transformed cells. However, the level of LM7 antigen expression did not correlate with the transcription level, and the LM7 gene was not silenced by DNA methylation in HeLa and 3T3 cells. In contrast, expression of the LM7 antigen was down-regulated during in vitro immortalization of both 3T3 and Chinese hamster ovary cells. The loss of LM-7 antigen expression was correlated with a dramatic decrease in the rate of proliferation, and it was reversible by blockade of DNA synthesis. This indicates that LM-7 antigen expression is dependent on the cell cycle and that it is modulated by genes active during the cell cycle. Therefore, the loss of LM-7 antigen expression is at the post-transcriptional level, and it suggests a direct involvement of the cell cycle in regulation of LM7 antigen expression.News
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