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We screened the TARDBP gene for mutations in 4 Japanese familial amyotrophic lateral sclerosis (ALS) families. Four patients (2 men, 2 women, mean age of onset: 54.3 years) in 3 ALS families with a family history of ALS had no mutations in the first exon and first intron of the TARDBP gene. Three mutations in the caspase 3 (CASP3) gene were detected in 3 of them. The first mutation A6C (p.Gly2Ala) was found in an ALS with minor family history. The second mutation F84L (p.Ala28Ser) and the third one (p.Leu51Pro) were present in an ALS with early onset. We concluded that CASP3 gene mutations account for approximately 2% of familial ALS.Density-functional theory/molecular mechanics: CCSD(T) level study of the gas phase S(N(2))-S(N(2))N reaction of ethane by ab initio (MP2) and all-electron calculations.
The S(N(2))-S(N(2))N reaction of ethane (C(2)H(6)) has been studied on the CCSD(T)-MP2 level, the only available treatment until now. The original, all-electron study of Sato et al. [J. Chem. Phys. 120,
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The report uses module HPA-8298 (how to use the HPA and the ICHG) to confirm the identity and compatibility of the lot.
Reagents and instruments used in this study were as follows.
L929 cells were used in the study.
L-glutamine was added to the culture media.
In all experiments, the test results were compared with a negative control using the same lot of the media (0.9% NaCl) and a positive control using a lot of media (DMEM) with Staphylococcus aureus, Ms.
an exponential growth phase (12 hours) in a shaker incubator at 37°C and atmospheric of 5% CO2.
In all experiments, the cells were harvested when the monolayer was more than 80% of confluency.
All chemicals were purchased from Sigma-Aldrich Inc., Singapore. The test results were analyzed using the Statistical Package for Social Sciences (SPSS) version 13. The variances were compared by the F-test. t-test was used for a one-way analysis of variance (ANOVA) of the data obtained. ANOVA was performed for the comparison of mean difference. The P-value of 0.05 was chosen as the criteria of statistical significance.
Results {#sec:results}
=======
The identifications of the microbial cultures used in this study are in the following:
– B.
– S.
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Assay Result & (Number of Lots, %) &\
& (20) & (1)\
Test results were within the acceptance criteria for all .
& &\
& (10) & (1)\
& (1) & (1)\
Test results for this study were analyzed as in the “[Assay results and risk interpretation”](#sec:results_and_risk_interpretation)”. The F-test revealed a difference between the control and bacterial counts for all lots tested as follows.
The t-test revealed that there was a statistically significant difference between the control and the bacterial count (y) with a 95% confidence level and at least 5% probability of type-I error.
The difference between the mean counts of the