Solutions Intermediate Teachers Book
Solutions Intermediate Teachers Book
Solutions Intermediate Teachers Book
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Enterococci have emerged as a leading cause of urinary tract infections worldwide. High resistance rates against ampicillin, cephalosporins, and quinolone antibiotics are frequently encountered among enterococci recovered from clinical specimens in Turkey. In this study, ceftiofur resistance isolates were determined from different hospitals of the eastern and northern regions in Turkey. Among 130 enterococci isolates, 11 were determined as resistant to ceftiofur with minimum inhibitory concentration levels of 4 μg/ml. These resistant isolates were identified as Enterococcus faecium by genotyping techniques. The resistance gene sequences of fsrA, cfr, erm(B), erm(C), and vatA were detected with primers for the tetracycline, macrolide, lincosamide, streptogramin, and fluoroquinolone resistance genes, respectively. The aminoglycoside resistance gene targets, rmtB, arr, acc, and ant(6)-Ib, were identified with polymerase chain reaction. Further identification of the fsr(A) gene in the 11 isolates was completed by sequencing. The results demonstrated the presence of three different genetic platforms of fsr(A) within the 11 isolates. The main resistance mechanisms were reported as cfr-cfrB, cfr-cfrR, cfr-cfrZ, fsr(A)-fsrB, and cfr-erm(B). All of the 11 isolates harbored the cfr genes that were transferred as a conjugative plasmid. This is the first report from Turkey that describes the occurrence of ceftiofur-resistant enterococci in the eastern and northern regions of Turkey. Antibiotic resistance in enterococci is a worldwide problem that is often associated with multiple resistances. This study reports the first detection of ceftiofur-resistant enterococci among Turkish isolates from different hospital locations.Q:
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