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Oligonucleotide probes were immobilized on carboxyl-terminal modified cellulose fibers with the DBA-Quadruplex method. The fiber probes were prepared by a simple immersion method using the carboxyl-terminal cellulose fibers; they were applied for DNA detection. In the case of DNA detection using the above fiber probes, no significant background noise was observed. Specially, the fiber probes were very useful for detecting multiple unknown DNAs from polymerase chain reaction (PCR) samples in a hybridization/washing process.The present invention relates to a method for the generation of antibodies in vivo against TNF-alpha, and more specifically to a method for the generation of antibodies in vivo against rat TNF-alpha.
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